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callispheres载药微球在递送奥沙利铂过程中的形态变化、负载力及释放特征:一项体外研究-ag捕鱼平台

文字: 2019-11-13    浏览次数:595    

objectives: this study aimed to explore the morphology, loadability, and releasing profiles of callispheres microspheres in delivering oxaliplatin.

目的:本研究旨在探讨callispheres载药微球在递送奥沙利铂过程中的形态变化、负载力以及释放特性。

 

methods: varied amount (20, 40, 60, and 80 mg oxaliplatin) and concentration (1.25,2.5, 5.0 mg/ml oxaliplatin) of oxaliplatin were mixed with callispheres microspheres with 3 sizes (50-150 μm, 100-300 μm, and 300-500 μm) to measure the loadability. of all, 20 mg oxaliplatin-loaded callispheres microspheres with 3 sizes was prepared to measure the releasing profiles, meanwhile, fetal bovine serum was added to determine the effect of serum on oxaliplatin releasing. the morphology and size distribution of callispheres microspheres with 3 sizes before and after 20 mg oxaliplatin loading were detected.

方法:将不同含量(20406080mg)和浓度(1.252.55.0 mg/ml)的奥沙利铂混合于3种规格的callispheres载药微球(50-150 μm100-300 μm 300-500 μm)以测量负载力。制备3种规格载有20 mg奥沙利铂的callispheres载药微球,测定其释放特性,同时加入胎牛血清以测定血清对奥沙利铂释放的影响。检测负载20mg奥沙利铂前后3种不同规格callispheres载药微球的形态和大小变化特征。

 

results: oxaliplatin amount was negatively correlated with loading efficiency with highest loadability in 20 mg oxaliplatin group (maximum 40% in 50-100 µm callispheres microspheres, 52% in 100-300 µm callispheres microspheres, and 52% in 300-500 µm callispheres microspheres), while oxaliplatin concentration was positively associated with loading efficiency. similar drug-releasing profiles were observed among oxaliplatin-loaded callispheres microspheres with 3 sizes, and a rapid drug release was discovered in callispheres microspheres with 3 sizes as well. we also found that fetal bovine serum did not affect the drug-releasing profiles of oxaliplatin-loaded callispheres microspheres. in addition, callispheres microspheres was modified a little to ellipse shape and less smooth after oxaliplatin loading, and it was enlarged to some extent.

结果:负载力与奥沙利铂的含量呈负相关,20 mg奥沙利铂组的负载率最高(50-100 µm100-300 µm300-500 µm callispheres载药微球最大负载率分别为40%52%52%);而负载力与奥沙利铂的浓度呈正相关。三种不同规格的载有奥沙利铂的callispheres载药微球具有相似的药物释放特征,并且能使药物快速释放。我们还发现胎牛血清不影响载有奥沙利铂的callispheres载药微球的药物释放。此外,加载奥沙利铂后,callispheres载药微球略微变为椭圆状,光滑度下降,并有一定程度上的增大。

 

conclusion: this study discloses drug loadability, releasing profiles, and morphology change of callispheres microspheres for delivering oxaliplatin, which provides potential evidences for application of oxaliplatin-loaded drug-eluting beads in clinical practice.

结论:本研究揭示了载有奥沙利铂的callispheres载药微球的载药负载力、释放特征及形态变化,为奥沙利铂载药微球的临床应用提供了依据。